The Effect Of K2EDTA Blood Storage Time Toward Temperature Space, Hemoglobin Levels and Erythrocyte Morphology

Febriyanto, Tedy and Welkriana, Putri Widelia (2021) The Effect Of K2EDTA Blood Storage Time Toward Temperature Space, Hemoglobin Levels and Erythrocyte Morphology. Star Shine Medical Journal, 1 (2). pp. 37-43. ISSN 2797-1502

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Abstract

Venous blood mixed with anticoagulants is usually used for hematological examination, in order to blood material does not clot. Checking hemoglobin levels and erythrocyte morphology uses an anticoagulant, one of which is EDTA (ethylenediaminetetraacetate), as sodium or potassium salt. Most of the hospitals and clinical laboratories in Kota Bengkulu still have many checks that are not carried out immediately. Then, there are delays caused by the accumulation of samples, especially when there is a Medical Check-Up program for company or factory workers with a large number of workers. This research was an experimental study in a laboratory. The sample in this study were 39 Students level 2 and 3 Department of Medical Laboratory Technology of Poltekkes Kemenkes Bengkulu 2020. The treatments were in the form of K2EDTA blood count storage time of less than 1 hour and more than 3 hours on hemoglobin levels and erythrocyte morphology at room temperature. The results showed the mean hemoglobin level of less than 1 hour was 16.15 g / dL and the mean hemoglobin level for more than 3 hours was 14.87 g / dL. Erythrocyte morphological mean results of less than 1 hour and more than 3 hours are normal. The results of this study showed there is an effect of storage time of less than 1 hour and more than 3 hours of K2EDTA blood at room temperature on hemoglobin levels and erythrocyte morphology. It is recommended to conduct further research on the risk factors for the effect of K2EDTA blood storage at room temperature on erythrocyte morphology.

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